ELISpot Flex: Human Granzyme B (HRP)

ELISpot Flex: Human Granzyme B (HRP)

3486-2H

Publications: 55
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT28
Detection mAbMT8610, biotin
Enzyme conjugateStreptavidin-HRP

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Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human granzyme B using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human Granzyme B (HRP)
ApplicationELISpot
AnalyteGranzyme B
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.

Selecting the appropriate mAbs is key to effectively detecting human Granzyme B, a critical serine protease secreted by cytotoxic T cells and natural killer cells. This mAb guide describes which combination of our Granzyme B mAbs should be used in different immunoassays.

 

Application guide: ELISA

Our human granzyme B ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

GranzymeB_mAb_pair_ELISA

Application guide: ELISpot

Our human granzyme B ELISpot kits can be used to detect the important cytokine directly upon secretion by T cells.

Capture mAb: MT28, unconjugated
Detection mAb: MT8610, biotin

Granzyme B mAb pair ELISpot

Application guide: FluoroSpot

Our human granzyme B FluoroSpot kits can be used to detect the important cytokine directly upon secretion by T cells alongside other analytes in the multiplex format.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

granzyme B FluoroSpot mab pair

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human Granzyme B ELISA, ELISpot, and FluoroSpot kits in everything from vaccine development to SARS-CoV-2 research. You can find it all in our Publication database.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002
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